Tender For tender for limited tender invited by department of biochemistry for intramural project for mrna gene expression analysis--, human serum rna isolation kit, human tissue rna isolation kit, cdna reverse transcription kit, sybr green master mix for real time pcr (compatible with cfx 96 biorad and quant studio 5 rt-pcr), zbp 1 primers (pcr grade) for gene expression assay, ☐, caspase 1 primers (pcr grade) for gene expression assay, aim 2 primers (pcr grade) for gene expression assay, gasdermin a primers (pcr grade) for gene expression assay, il-17 primers (pcr grade) for gene expression assay, d., ribosomal protein large po (rplpo) primers (pcr grade) for gene expression assay
Tender For supply of fret-real time pcr (bio molecular analytics system) along with accessories - fret-real time pcr (bio molecular analytics system) along with accessories along with the accessories with three years warranty, automated cell counter with three years warranty, semidry rapid western transfer system, optional: reverse transcription supermix; 100x 20 l rxns 400 l; 4 x100 l of 5x supermix, containing reverse transcriptase; rnase inhibitor, dntps, primers, mgcl2, stabilizers; 50 reactions of no-rt control supermix, optional: high-fidelity dna polymerase, 100 u (2u/ l), 50 l,(stand-alone enzyme) that includes 5x reaction buffers, mgcl2 solution, dmso, optional: horizontal gel electrophoresis system for dna and rna, 7 x10 cm tray, with mini-gel caster, mini horizontal electrophoresis system that includes 8- and 15-well combs, 7 x 10 cm (w x l), uv-transparent tray, mini-gel caster, optional: rna isolation reagent 100 ml, reagent for dna-and protein-free rna isolation, optional: plasmid miniprep kit 100 preps; plasmid dna purification kit that includes spin columns and plasmid dna isolation solutions (cell resuspension and lysis, neutralization, wash), optional: molecular biology grade agarose: 125g general purpose agarose for nucleic acid electrophoresis which is certified free of inhibitors, dnases, rnases, optional: 1 year amc post warranty period