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Tender For supply of crs_surgical_ ph no.:319065_ occult blood (in stool) reagentstrips.[quantity tolerance (+/-): 5 %age , item category : normal , total po value variation permitted: max 8 lacs ] ]
Tender For purchase of chemicals - purchase of chemicals, diluent for dna extractionsize 500mla diluent used in dna extraction is typically te buffer (10 mm tris-hcl, 1 mm edta, ph ~8.0) or nuclease-free water, used to dissolve and dilute extracted dna. tris maintains a stable ph, while edta chelates divalent cations (e.g., mg ), thereby inhibiting dnase activity and protecting dna from degradation. the diluent must be sterile and free of nucleases to ensure the integrity of dna for downstream applications such as pcr, sequencing, and cloning., luria bertani mediumsize 500gluria bertani medium is a nutrient-rich growth medium commonly used for culturing bacteria such as escherichia coli and agrobacterium tumefaciens. it contains tryptone (10 g/l), yeast extract (5 g/l), and sodium chloride (10 g/l), which provide essential nutrients, vitamins, and osmotic balance for bacterial growth. lb medium supports rapid cell growth and is widely used in molecular biology for cloning, plasmid propagation, and transformation experiments. for solid media, agar (15g/l) is added., chloroform (high analytical reagent grade) size 2.5lchloroform (high analytical reagent grade) is a high-purity organic solvent used in dna extraction, particularly in phenol chloroform methods, to separate proteins and other contaminants from nucleic acids. it enhances phase separation by forming a distinct organic layer, allowing dna to remain in the aqueous phase. due to its toxicity and volatility, it must be handled under a fume hood with proper safety precautions., depcsize 25mldepc is a chemical used to inactivate rnases in water and laboratory solutions, making them suitable for rna work. it modifies histidine residues in proteins, thereby deactivating rnase enzymes that can degrade rna. depc-treated solutions are typically incubated and then autoclaved to remove residual depc, ensuring they are safe for use in downstream applications such as rna extraction and cdna synthesis., tb green master mixsize 200rnxtb green master mix is a ready-to-use reagent used in real-time pcr (qpcr) for dna amplification and detection. it contains tb green dye (a double-stranded dna-binding fluorescent dye), dna polymerase, dntps, mg , and reaction buffer. the dye binds to amplified double-stranded dna and emits fluorescence, allowing real-time monitoring of pcr amplification. it is commonly used for gene expression analysis and quantification of dna., dna loading dyesize 10 inchdna loading dye is a solution added to dna samples before agarose gel electrophoresis. it contains tracking dyes (such as bromophenol blue and xylene cyanol) that allow monitoring of dna migration during the run, and a dense agent (such as glycerol or sucrose) that helps the sample sink into the wells. it may also include edta to protect dna from nuclease degradation., metaloopa metal loop is a reusable laboratory tool made of platinum or nichrome wire, used for transferring and inoculating microorganisms onto culture media. it is sterilized by heating in a flame until red hot before and after use to prevent contamination. it is commonly used in microbiology for streaking plates and handling bacterial cultures.
Tender For purchase of chemicals - purchase of chemicals, t4 dna ligasequantity 01size 2000unitst4 dna ligase is an enzyme derived from bacteriophage t4 that catalyzes the formation of phosphodiester bonds between adjacent 3 -oh and 5 -phosphate ends of dna. it is widely used in molecular biology to join dna fragments, such as ligating insert dna into vectors during cloning. the enzyme requires atp as a cofactor and functions efficiently on both cohesive (sticky) and blunt-ended dna fragments., bsaiquantity 01size 1000unitsbsai is a type iis restriction endonuclease that recognizes the asymmetric dna sequence 5 -ggtctc-3 but cuts dna at a defined distance outside of its recognition site. this property allows it to generate custom overhangs, making it widely used in golden gate cloning for seamless and directional assembly of dna fragments. it requires mg as a cofactor and functions optimally under specific buffer and temperature conditions (typically 37 c)., acetosyringonequantity 01size 5gacetosyringone is a phenolic compound used in plant transformation protocols, particularly with agrobacterium tumefaciens. it induces the expression of virulence (vir) genes in the bacterium, which are essential for t-dna transfer into plant cells. it is commonly added to infection and co-cultivation media to enhance transformation efficiency and is typically dissolved in solvents like ethanol or dmso for preparation., sodium hydroxide pelletsquantity 01size 500glsodium hydroxide pellets are a strong alkaline reagent used in molecular biology for ph adjustment and cell lysis during dna extraction protocols. they help denature dna and proteins under alkaline conditions, as in alkaline lysis methods for plasmid isolation. naoh is highly caustic and hygroscopic, so it must be handled with care and stored in tightly sealed containers to prevent moisture absorption., 5. ctabquantity 01size 100glctab is a cationic detergent widely used in plant dna extraction protocols. it disrupts cell membranes and forms complexes with polysaccharides, allowing their separation from dna. ctab helps obtain high-quality dna by removing contaminants such as polysaccharides and some proteins, making it especially useful for plant tissues rich in secondary metabolites.
Tender For supply of fast start essential dna probes master 10 multiply 5 m for upto 5000 reactions of 20 ul final volume , light cycler 480 multiwell plate 96, white,50 plates with sealing foil , nitrile powder free gloves medium , nitrile powder free gloves small , ethyl alcohol 500 ml , sterile racked filter tips 10 ul , aerosol barrier tips , 2 point 0 ml microcentrifuge tubes , dneasy blood and tissue kit for dna isolation 250 reaction , nucleospin gel and pcr clean up upto 50 reaction , marker pen fine tip blue , marker pen fine tip red , marker pen fine tip black , test tube brush , pointed forceps 5 inch , minute pin length 15 mulitply 0 point 10 diameter , eppendorf tube 2 ml , entomological needle 13 point 5 cm , eppendorf tube stand 2 ml , lab soaker , dipper enamel , pipette plastic 5 ml , pipette plastic 10 ml , rubber teat for 10 ml pipette , pasteur pipette glass 3 ml with rubber teat , forceps , minute pin no 2 , eppendorf tube 1 point 5 ml , pyrethrum extract , dneasy blood and tissue kit 250 reaction , rneasy mini kit 250 reaction , taqman tm multiplex master mix 5 ml , reliance one step multiplex rt- qpcr supermix, 200 multiply 20rxns, 1 ml , 0 point 2 ml 8 tube pcr strips without caps,low profile,white , 0 point 2 ml 8 tube pcr strips without caps,optical,ultraclear , promega gel loading dye 6x , ethanol,absolute molecular biology grade 500 ml , premium grade ethanol 100 percent 500 ml , 1 point 5 ml tube , 0 point 2 ml tube , 2 ml tube , tips 2 to 10 ul filter tips , tips 2 to 20 ul filter tips , tips 20 to 200 ul filter tips , tips 200 to1000 ul filter tips , rnaseaway decontimation reagent 250 ml , asn salt 5 g , lb agar 500 g , 1m iptg , lb broth 500 g , kanamycin sulphate 1 g , tetracycline 1 g , calcium anhydrous chloride 500 g , carbenicillin disodium salt 1 g , storage box for 1 point 5 ml microtubes, pp clear type,100 holes , sodium hypochlorite, hi ar tm acs, 4 percent w v solution ) /bid number : gem/2026/b/7593707 * /dated: 27-05-2026 & & / bid document 1 / 45