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Tender For rate contract for supply of consumables/non-consumables forrvrdl, dept. of microbiology - item description name of the item (pack size wherever mentioned should strictly adherence the other pack size will not be entertained) rate to be quoted only for the first quality, real time pcr mastermix for dna viruses universal qpcr master mix compatible with taqman probes at 2x concentration should have hot start taq dna polymerase nuclease free water and necessary sample buffers should be provided sample must be given for a minimum of 50 reactions, one step real time pcr mastermix for rna viruses one step reverse transcription real time pcr master mix (5x) compatible with taqman probes (multiplexing) rt enzyme high fidelity hot start taq dna polymerase nuclease free water and necessary sample buffers should be provided sample must be given for a minimum of 50 reactions, one step real time pcr mastermix for rna viruses one step reverse transcription real time pcr master mix (2x) compatible with taqman probes, *rt enzyme, *hot start taq dna polymerase *nuclease free water and necessary sample buffers should be provided sample must be given for a minimum of 50 reactions, cdna synthesis kit first strand cdna synthesis kit containing reverse transcriptase rnase inhibitor dntp mix oligo dt primer random hexamer primer, and buffer, hotstart conventional pcr mastermix conventional hot start pcr master mix should be colored- ready to load directly from pcr tube to gel sample must be given for a minimum of 50 reactions, pcr product gel purification kit (spin column) spin column-based pcr gel purification kit with necessary buffers, pcr product purification kit (spin column) spin column-based direct pcr product purification kit with necessary buffers, molecular grade agarose for gel electrophoresis molecular grade low eeo, tris acetate edta buffer (10x) for gel electrophoresis application: electrophoresis, tris edta buffer (1x), rnase, dnase free molecular grade 100 ml molecular grade dnase, rnase-free ready to use for reconstitution of primers, nucleic acid extraction plasticware compatible for ils genfast automated nucleic acid extraction machine (4 nos of 96 position -deep well plates, 1 comb plate, 1 comb nucleic acid extraction must provide compatibility certificate from oem u bottom sample must be given for a minimum of 3*96 reactions, real time pcr plates -cobas z480 qpcr plates: opaque semi skirted, low profile 0.1ml volume compatible with cobas z480 machine and cfx96 should be provided with clear sealing film for real time pcr applications should be dnase, rnase, and pyrogen free must produce compatibility certificate from oem should provide 5 sample plates, real time pcr plates - qs5 qpcr plates: ultra clear, * semi skirted, regular profile * 0.2ml volume, * compatible with qs5, *should be provided with clear sealing film for real time pcr applications * must produce compatibility certificate from oem * should be dnase, rnase, and pyrogen free sample should be given for 5 plates, real time pcr strips -cobas z480 qpcr strips: opaque, low profile * 8well per strip, * must provide transparent strip caps * compatible with cobas z480 real time pcr and cfx 96* must produce certificate from oem* should be dnase, rnase, and pyrogen free sample should be given for 5 strips, real time pcr strips qs5 qpcr strips: ultra clear, regular profile 8well per strip attached with transparent caps compatible with qs5 real time pcr must produce certificate from oem should be dnase, rnase, and pyrogen free sample should be given for 5 strips, sanger sequencing services sequencing service for 1 sample includes pcr product purification, both forward and reverse strand sequencing, 100ul filter tips filter tips 100ul universal tips, must be compatible with standard pipettes brands including eppendorf, thermofisher, gilson and tarson., 10ul filter tips filter tips 10-20ul universal tips, must be compatible with standard pipettes brands including eppendorf, therm
Tender For supply of point machine and various other items as per boq attached inspection rdso rites consignee. - supply of point machine irs type 220 mm stroke clamp type as per irs s$-24/2002 (amendment 1) or latest.(inspection agency:- rdso), supply of q-series led ecr (universal type i.e.qecx-61), 4f/4b complete with plug board(base plate), connectors, retaining clips etc. and conforming to brs: 941a sts / e / relays / ac lit led signal / 09 - 2002, amdt.-1 or latest and rdso spec.no.irs: s - 34, s- 23 or latest, as applicable. the interlocking code for this unit shall be "abdhk". (inspection by rdso)., contactor unit (qbca1 type), 24 v dc, 2hf / 4b complete with plug board (base plate), connectors, retaining clips etc and conforming to brs- 943 and rdso specn. no. irs : s - 34, s- 23, s - 60 or latest, as applicable. th e interlocking code for this unit shall be "bcejk". (inspection by rdso)., q-series magnetically latched neutral line relay (ql1 type), 11f / 4b, 24v dc complete with plug board (base plate), connectors and retaining clips and con forming to brs-935a and rdso specn. no.irs : s - 34, s - 23 or latest, as applicable. the interlocking code for this unit shall be "abdeg.(inspection by rdso)., supply of basic material to construct unit maintenance free earth as per rdso specs. no. rdso/spn/197 ver. 1.0 or latest to achieve a resistance less than 1 ohm. it consist of following:(a) copper bonded steel electrode of minimum 3.0 meter long, minimum 17.0 mm dia with copper bonding thickness of minimum 250 microns and ul listed and marked = 1 no. (b) earth enhancement material supplied in sealed bags of minimum 10 kg = 3 nos. (c) copper strip of 150x25x6 mm, to terminate earth rod = 1 no. (d) copper strip of 300x25x6 mm (meeb) = 1 no. (e) copper strip of 150x25x6mm (seeb) = 1 no. (ii) supply of 35 sq. mm multi strand single core pvc insulated copper cable as per is: 694 for connecting main earth electrode to meeb in the equipment room in duplicate. (minimum 10 meter.) (iii) supply of 16 sq. mm multi strand single core pvc insulated copper cable as per is: 694 for connecting meeb to seeb and spd to meeb (minimum 10 meter.) (iv) supply of 10 sq. mm multi strand single core pvc insulated copper cable as per is: 694 for connecting various equipments to seeb (v) supply of copper lug sleeve for 3/16 cable.(inspection by consignee)., supply of master telephone with voice logging facility for electronic lc gate telephone system as per specification no: rdso/spn/tc-51/2011 revision 3.0 with amendment no. 1 or latest. (inspection by rites)., electronic lc gate slave telephone system with voice recorder as per rdso specification no. rdso/spn/ tc/51/2021 revision 1.0 (inspection agency:- consignee), supply of centralized power supply system 24 volts dc power pack with 7ah mf battery for electronic lc gate telephone system as per rdso spec:- no. rdso / spn / tc / 51 / 2011 (rev. 3.0) amdt - 1 or latest.(inspection agency:- consignee), supply of optical power meter, type-b, as per tec spec. gr / opm - 01 / 03 feb. 2005 or latest. (make & model khushicom-k280) or similar branded make. (inspection by consignee)., supply and testing of optical laser light source of make- jdsu model- ols-35 or similar branded make.(inspection agency:- rites), supply of optical laser torch of reputed make. (make & model-proskit mt-7530) or similar branded make. (inspection agency: - consignee), supply of automatic fusion splicer for single mode optical fibre cable, along with accessories as per tec spec. tec/gr/tx/osm 001/04/sep-12 or latest of make superior/fujikura or similar. consignee shall approve the supply only after successful demonstration at site. (inspection agency:- rites), supply of otdr suitable for 1310/1550nm fiber with resolution of 60 cm, dynamic range of 314/29 db with all accessories for working on single mode optical fiber as per tec spec. tec/gr/tx/otd 001/05/sep-19 or latest, of reputed make like yokogawa/zdsu/viavi or similar. consi