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Tender For purchase of chemicals - purchase of chemicals, diluent for dna extractionsize 500mla diluent used in dna extraction is typically te buffer (10 mm tris-hcl, 1 mm edta, ph ~8.0) or nuclease-free water, used to dissolve and dilute extracted dna. tris maintains a stable ph, while edta chelates divalent cations (e.g., mg ), thereby inhibiting dnase activity and protecting dna from degradation. the diluent must be sterile and free of nucleases to ensure the integrity of dna for downstream applications such as pcr, sequencing, and cloning., luria bertani mediumsize 500gluria bertani medium is a nutrient-rich growth medium commonly used for culturing bacteria such as escherichia coli and agrobacterium tumefaciens. it contains tryptone (10 g/l), yeast extract (5 g/l), and sodium chloride (10 g/l), which provide essential nutrients, vitamins, and osmotic balance for bacterial growth. lb medium supports rapid cell growth and is widely used in molecular biology for cloning, plasmid propagation, and transformation experiments. for solid media, agar (15g/l) is added., chloroform (high analytical reagent grade) size 2.5lchloroform (high analytical reagent grade) is a high-purity organic solvent used in dna extraction, particularly in phenol chloroform methods, to separate proteins and other contaminants from nucleic acids. it enhances phase separation by forming a distinct organic layer, allowing dna to remain in the aqueous phase. due to its toxicity and volatility, it must be handled under a fume hood with proper safety precautions., depcsize 25mldepc is a chemical used to inactivate rnases in water and laboratory solutions, making them suitable for rna work. it modifies histidine residues in proteins, thereby deactivating rnase enzymes that can degrade rna. depc-treated solutions are typically incubated and then autoclaved to remove residual depc, ensuring they are safe for use in downstream applications such as rna extraction and cdna synthesis., tb green master mixsize 200rnxtb green master mix is a ready-to-use reagent used in real-time pcr (qpcr) for dna amplification and detection. it contains tb green dye (a double-stranded dna-binding fluorescent dye), dna polymerase, dntps, mg , and reaction buffer. the dye binds to amplified double-stranded dna and emits fluorescence, allowing real-time monitoring of pcr amplification. it is commonly used for gene expression analysis and quantification of dna., dna loading dyesize 10 inchdna loading dye is a solution added to dna samples before agarose gel electrophoresis. it contains tracking dyes (such as bromophenol blue and xylene cyanol) that allow monitoring of dna migration during the run, and a dense agent (such as glycerol or sucrose) that helps the sample sink into the wells. it may also include edta to protect dna from nuclease degradation., metaloopa metal loop is a reusable laboratory tool made of platinum or nichrome wire, used for transferring and inoculating microorganisms onto culture media. it is sterilized by heating in a flame until red hot before and after use to prevent contamination. it is commonly used in microbiology for streaking plates and handling bacterial cultures.
Tender For corrigendum : sch 5 drugs, pharmaceutical and disinfectants by tender - schedule v (drugs, pharmaceuticals & disinfectant), carbolic (phenol crystal) acid, ammonium chloride, borax, crystal violet medicinal use, emulsifying wax, ether solvent, methyl salicylate, paraffin soft white, potassium chloride, potassium iodide, soda lime granules medical grade for anaesthesia purpose. non-hygroscopic . granules size 4-8 mesh size. suitable indication with change of colour to denote exhaustion of granules. (rate to be quoted for 1 x 5 kg tin) i.p., sodium bicarbonate, calamine prepared, clove oil, soap solution i. s. i. mark, antiseptic solution containing benzalkonium chloride solution 20% w/v equivalent to benzalkonium chloride equivalent to 10%w/v, 70% to 80% alcohol based surgical dry hand antiseptic with 0.5% to 1% chlorhexidine gluconate and containing suitable emollient and moisturizer., 70% to 80% alcohol based surgical dry hand antiseptic with a substance included in i.p.other than chlorhexidine gluconate having a broad spectrum activity against bacteria, virus (inclusive hiv and hbv) and fungi with suitable emollient and moisturizer., chlorhexidine (not less than 1.5% w./ v. or 7.5 % v/v) with cetrimide 15%, povidone iodine i.p. should contain povidone iodine 10% (available iodine 1% w/v) (rate to be quoted for 1000ml), solution containing > 2 % w/v glutaraldehyde with a powder activator free of surfactants with stable alkaline ph for 14 days with concentration detections strips(10 strips/5lit jar.), surface & environmental disinfectant each 100 gm containing 1.6 dihydroxy, 2.5 diohexane 11.2gm, gluteraldehyde 5gm benzalkonium chloride 5gm alkyl urear derivatives 3.00gm ( effectivity reports against hbv/hiv & t.b. is necessary), surface and environmental disinfectant: aldehyde free disinfec- tant with bacetericidal, fungicidal, virucidal(including hiv and hbv) and sporicidal activity capable of being used as both surface and environmental disinfectant as well as for fogging. the product label should mention the shelf life also., sodium chloride (i.p.), opa orthophthalaldehyde 0.55% w/w with marker technology with detection strips (5 test strips/5 lit pack), enzymatic instrument cleaner multienzymatic enzymatic cleaner, neutral ph, compatible for use on surgical, medical and dental instruments with atleast protease ,amylase,& lipase enzyme activity (rate to be quoted per ltr 1 x 1 ltrs) (supply in suitable packing upto 5 lit. jar), denaturated spirit 95-97% ordinary, analgesic transdermal patch fentanyl 25mcg/hr, adhesive remover spray 100 % silicon, 1 % acetic acid 500 ml bottle, methylene blue, na fluorescence strips, molecular grade water, lint free tissue
Tender For auction sale of depot miscellaneous scrap - 98414501 different types of e-waste items such as different types & makes of computers & its accessories/ peripherals, tabs/laptop, monitors, ups, mps, speakers, printers, cpu, hard disc, key board, mouse, toners, cartridges, scanners, projectors, terminals, servers, electronic led cards, pcb card, cpu card, driver card, indicator card, xerox machine, plotter, fax machine, ribbons, thin client, fire wall, routers, plotter, lan extender, risograph, body warn camera, spike guard, typewriter, calculators,cd/dvd drive, remote, static mux and other computer related items scrap, display board, released off sorts & sizes, broken and damaged. un serviceable to railway use. loading by purchaser. (note: cpcb mpcb,pcc license to be produced at the time of delivery).note: loose plastic/ms parts released from these items is part of lot to be lifted during delivery