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Tender For purchase of chemicals - purchase of chemicals, diluent for dna extractionsize 500mla diluent used in dna extraction is typically te buffer (10 mm tris-hcl, 1 mm edta, ph ~8.0) or nuclease-free water, used to dissolve and dilute extracted dna. tris maintains a stable ph, while edta chelates divalent cations (e.g., mg ), thereby inhibiting dnase activity and protecting dna from degradation. the diluent must be sterile and free of nucleases to ensure the integrity of dna for downstream applications such as pcr, sequencing, and cloning., luria bertani mediumsize 500gluria bertani medium is a nutrient-rich growth medium commonly used for culturing bacteria such as escherichia coli and agrobacterium tumefaciens. it contains tryptone (10 g/l), yeast extract (5 g/l), and sodium chloride (10 g/l), which provide essential nutrients, vitamins, and osmotic balance for bacterial growth. lb medium supports rapid cell growth and is widely used in molecular biology for cloning, plasmid propagation, and transformation experiments. for solid media, agar (15g/l) is added., chloroform (high analytical reagent grade) size 2.5lchloroform (high analytical reagent grade) is a high-purity organic solvent used in dna extraction, particularly in phenol chloroform methods, to separate proteins and other contaminants from nucleic acids. it enhances phase separation by forming a distinct organic layer, allowing dna to remain in the aqueous phase. due to its toxicity and volatility, it must be handled under a fume hood with proper safety precautions., depcsize 25mldepc is a chemical used to inactivate rnases in water and laboratory solutions, making them suitable for rna work. it modifies histidine residues in proteins, thereby deactivating rnase enzymes that can degrade rna. depc-treated solutions are typically incubated and then autoclaved to remove residual depc, ensuring they are safe for use in downstream applications such as rna extraction and cdna synthesis., tb green master mixsize 200rnxtb green master mix is a ready-to-use reagent used in real-time pcr (qpcr) for dna amplification and detection. it contains tb green dye (a double-stranded dna-binding fluorescent dye), dna polymerase, dntps, mg , and reaction buffer. the dye binds to amplified double-stranded dna and emits fluorescence, allowing real-time monitoring of pcr amplification. it is commonly used for gene expression analysis and quantification of dna., dna loading dyesize 10 inchdna loading dye is a solution added to dna samples before agarose gel electrophoresis. it contains tracking dyes (such as bromophenol blue and xylene cyanol) that allow monitoring of dna migration during the run, and a dense agent (such as glycerol or sucrose) that helps the sample sink into the wells. it may also include edta to protect dna from nuclease degradation., metaloopa metal loop is a reusable laboratory tool made of platinum or nichrome wire, used for transferring and inoculating microorganisms onto culture media. it is sterilized by heating in a flame until red hot before and after use to prevent contamination. it is commonly used in microbiology for streaking plates and handling bacterial cultures.
Tender For supply and delivery of physical and chemical parameters, lab chemicals at head water works of rajamahendravaram municipal corporation. - ???????a buffer solution 500 ml, ammonium purpurate (murexide) sq 5 gm, bromocresol green indicator 125ml, chemicals, cobaltous chloride hexahydrate purified 500gm, conductivity standard 1413 make: thermo scientific capacity: 480ml, dropping bottles 125ml, duracells batteries 1.5v, ec electrode, edta disodium salt er 500 gm, eriochrome black t 25gm, flocculator (jar test apparatus) for six stirrers (digital lcd based micro controller) make: savant technical specification: flocculator has been specially designed for use in water treatment plants to correctly estimate the dosing of alum and such other coagulants for treatment of water and sewage. in provides identical test conditions for all samples. illumination base consists of fluorescent tube mounted below translucent plastic plate to provide diffused cold light through floc samples. flocculator consists of heavy-duty dc driven variable speed motor having speed from 20 to 180 rpm with built in speed control. stainless steel stirring rods are provided with adjustable spacers to adjust the depth of stirring paddies. the stirring shaft can be removed without disturbing other stirrers. this unit is supplied without beakers to work on 220 volts a.c., hydrachloric acid 35-38% (1.18) 500ml, isopropylalacohal 500ml, ph 10.01 buffer solution make: thermo scientific capacity: 480ml, ph 4.01 buffer solution make: thermo scientific capacity: 480ml, ph 7.00 buffer solution make: thermo scientific capacity: 480ml, ph electrode (rangs 0-14), phenolphthalein indicator solution 125 ml, potassium chloride 500grms, potassium chloroplatinate (iv) 5gm, potassium chromate er 500gm, reagents visocolor powder pillows iron photometric test part number: 936227 make: mn measuring range: iron 0.03-3.00 mg/l fe number of tests: 100, reagents visocolor powder pillows nitrate photometric test part number: 936226 make: mn measuring range nitrate - 5-220 mg/l no, number of tests: 100, s.no, silver nitrate er 500ml, sodium carbonate anhydrous er 500 gm, sodium hydroxide pellets 98% 500gm, stabilizer input voltage: 90 vac-290 vac make: reputed, standard test nanocolor chloride part number: 931218 make: mn measuring range chloride 0.2-125 mg/l ci number of tests: 250, standard test nanocolor fluoride part number: 931227 make: mn measuring range fluoride -0.05-2.00 mg/l f number of tests: 500, sulphuric acid er 500 ml, tissue rolls, titrimetric test kit visocolor eco total hardness make: mn, germany model: 931029 gradation: total hardness - 1 drop correspondes to 1.25 °e method: total hardness-complexometric titration no of test: 110 test(s), titrimetric test kit visocolor he alkalinity part number: 915007 make: mn measuring range alkalinity-0.2-7.2 mmol/l. oh (1 syringe filling) number of tests: 200, tube test nanocolor total nitrite part number: 936230 make: mn measuring range 0.01-0.30mg/l number of tests: 100