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Tender For rate contract for supply of consumables/non-consumables forrvrdl, dept. of microbiology - item description name of the item (pack size wherever mentioned should strictly adherence the other pack size will not be entertained) rate to be quoted only for the first quality, real time pcr mastermix for dna viruses universal qpcr master mix compatible with taqman probes at 2x concentration should have hot start taq dna polymerase nuclease free water and necessary sample buffers should be provided sample must be given for a minimum of 50 reactions, one step real time pcr mastermix for rna viruses one step reverse transcription real time pcr master mix (5x) compatible with taqman probes (multiplexing) rt enzyme high fidelity hot start taq dna polymerase nuclease free water and necessary sample buffers should be provided sample must be given for a minimum of 50 reactions, one step real time pcr mastermix for rna viruses one step reverse transcription real time pcr master mix (2x) compatible with taqman probes, *rt enzyme, *hot start taq dna polymerase *nuclease free water and necessary sample buffers should be provided sample must be given for a minimum of 50 reactions, cdna synthesis kit first strand cdna synthesis kit containing reverse transcriptase rnase inhibitor dntp mix oligo dt primer random hexamer primer, and buffer, hotstart conventional pcr mastermix conventional hot start pcr master mix should be colored- ready to load directly from pcr tube to gel sample must be given for a minimum of 50 reactions, pcr product gel purification kit (spin column) spin column-based pcr gel purification kit with necessary buffers, pcr product purification kit (spin column) spin column-based direct pcr product purification kit with necessary buffers, molecular grade agarose for gel electrophoresis molecular grade low eeo, tris acetate edta buffer (10x) for gel electrophoresis application: electrophoresis, tris edta buffer (1x), rnase, dnase free molecular grade 100 ml molecular grade dnase, rnase-free ready to use for reconstitution of primers, nucleic acid extraction plasticware compatible for ils genfast automated nucleic acid extraction machine (4 nos of 96 position -deep well plates, 1 comb plate, 1 comb nucleic acid extraction must provide compatibility certificate from oem u bottom sample must be given for a minimum of 3*96 reactions, real time pcr plates -cobas z480 qpcr plates: opaque semi skirted, low profile 0.1ml volume compatible with cobas z480 machine and cfx96 should be provided with clear sealing film for real time pcr applications should be dnase, rnase, and pyrogen free must produce compatibility certificate from oem should provide 5 sample plates, real time pcr plates - qs5 qpcr plates: ultra clear, * semi skirted, regular profile * 0.2ml volume, * compatible with qs5, *should be provided with clear sealing film for real time pcr applications * must produce compatibility certificate from oem * should be dnase, rnase, and pyrogen free sample should be given for 5 plates, real time pcr strips -cobas z480 qpcr strips: opaque, low profile * 8well per strip, * must provide transparent strip caps * compatible with cobas z480 real time pcr and cfx 96* must produce certificate from oem* should be dnase, rnase, and pyrogen free sample should be given for 5 strips, real time pcr strips qs5 qpcr strips: ultra clear, regular profile 8well per strip attached with transparent caps compatible with qs5 real time pcr must produce certificate from oem should be dnase, rnase, and pyrogen free sample should be given for 5 strips, sanger sequencing services sequencing service for 1 sample includes pcr product purification, both forward and reverse strand sequencing, 100ul filter tips filter tips 100ul universal tips, must be compatible with standard pipettes brands including eppendorf, thermofisher, gilson and tarson., 10ul filter tips filter tips 10-20ul universal tips, must be compatible with standard pipettes brands including eppendorf, therm
09 Feb, 2026
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