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Tender For purchase of chemicals - purchase of chemicals, diluent for dna extractionsize 500mla diluent used in dna extraction is typically te buffer (10 mm tris-hcl, 1 mm edta, ph ~8.0) or nuclease-free water, used to dissolve and dilute extracted dna. tris maintains a stable ph, while edta chelates divalent cations (e.g., mg ), thereby inhibiting dnase activity and protecting dna from degradation. the diluent must be sterile and free of nucleases to ensure the integrity of dna for downstream applications such as pcr, sequencing, and cloning., luria bertani mediumsize 500gluria bertani medium is a nutrient-rich growth medium commonly used for culturing bacteria such as escherichia coli and agrobacterium tumefaciens. it contains tryptone (10 g/l), yeast extract (5 g/l), and sodium chloride (10 g/l), which provide essential nutrients, vitamins, and osmotic balance for bacterial growth. lb medium supports rapid cell growth and is widely used in molecular biology for cloning, plasmid propagation, and transformation experiments. for solid media, agar (15g/l) is added., chloroform (high analytical reagent grade) size 2.5lchloroform (high analytical reagent grade) is a high-purity organic solvent used in dna extraction, particularly in phenol chloroform methods, to separate proteins and other contaminants from nucleic acids. it enhances phase separation by forming a distinct organic layer, allowing dna to remain in the aqueous phase. due to its toxicity and volatility, it must be handled under a fume hood with proper safety precautions., depcsize 25mldepc is a chemical used to inactivate rnases in water and laboratory solutions, making them suitable for rna work. it modifies histidine residues in proteins, thereby deactivating rnase enzymes that can degrade rna. depc-treated solutions are typically incubated and then autoclaved to remove residual depc, ensuring they are safe for use in downstream applications such as rna extraction and cdna synthesis., tb green master mixsize 200rnxtb green master mix is a ready-to-use reagent used in real-time pcr (qpcr) for dna amplification and detection. it contains tb green dye (a double-stranded dna-binding fluorescentdye), dna polymerase, dntps, mg , and reaction buffer. the dye binds to amplified double-stranded dna and emits fluorescence, allowing real-time monitoring of pcr amplification. it is commonly used for gene expression analysis and quantification of dna., dna loading dyesize 10 inchdna loading dye is a solution added to dna samples before agarose gel electrophoresis. it contains tracking dyes (such as bromophenol blue and xylene cyanol) that allow monitoring of dna migration during the run, and a dense agent (such as glycerol or sucrose) that helps the sample sink into the wells. it may also include edta to protect dna from nuclease degradation., metaloopa metal loop is a reusable laboratory tool made of platinum or nichrome wire, used for transferring and inoculating microorganisms onto culture media. it is sterilized by heating in a flame until red hot before and after use to prevent contamination. it is commonly used in microbiology for streaking plates and handling bacterial cultures.
Tender For purchase of chemicals - purchase of chemicals, petri dishes size 90mmpetri dishes are shallow, circular laboratory containers made of glass or disposable plastic, used for culturing microorganisms and plant tissues on solid media such as agar. they provide a sterile surface for growth, observation, and isolation of colonies, and are widely used in microbiology and molecular biology experiments., d-(+)-maltose quantity 5size 1kgd-(+)-maltose is a disaccharide composed of two glucose units linked by an -1,4 glycosidic bond. it is used in microbiology as a carbon and energy source in culture media to support the growth of microorganisms and in biochemical assays for studying carbohydrate metabolism., ampicillinsize 25gampicillin is a -lactam antibiotic used in molecular biology to select bacteria carrying plasmids with the ampicillin resistance (ampr) gene. it inhibits bacterial cell wall synthesis by targeting penicillin-binding proteins, leading to cell lysis. it is commonly added to culture media to maintain selective pressure during cloning and plasmid propagation., sucrose size 500gsucrose is a disaccharide composed of glucose and fructose, widely used in molecular biology and plant tissue culture as a carbon and energy source. it also acts as an osmotic stabilizer, helping maintain cell integrity and proper osmotic balance in media and solutions. sucrose is commonly included in plant transformation and regeneration media to support cell division, growth, and differentiation, and in certain protocols it is used to protect cells during handling and processing., l-prolinesize 25gl-proline is an amino acid commonly used in plant tissue culture and molecular biology as an osmoprotectant and stress-related metabolite. it helps stabilize proteins and cellular structures under stress conditions and can enhance cell growth, regeneration, and transformation efficiency. l-proline is often added to culture media to improve callus induction and support plant cell development., triton x-100size 100mltriton x-100 is a non-ionic detergent widely used in molecular biology for cell lysis and membrane permeabilization. it solubilizes lipids and membrane proteins without denaturing proteins, making it useful in dna and protein extraction protocols. it is also used in washing buffers to reduce non-specific interactions during experimental procedures.